These blockers are single purified proteins that provide blocking without compromising sensitivity. Try more flexible western blot blockers like StartingBlock Blocking Buffer and SuperBlock Blocking Buffer. It provides the highest-level sensitivity and better signal-to-noise ratios than other commercially available high-performance HRP substrate with exposures within a fraction of the time. Want a better way? Try SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate, an ultrasensitive enhanced chemiluminescent (ECL) substrate that enables high attogram level protein detection with horseradish peroxidase (HRP) conjugated antibodies. Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits. However, because 5% non-fat milk is a strong blocker, it can over block and suppress signal. 5% non-fat milk is a strong blocker and a great choice for high abundant proteins or when there is non-specific binding from your primary antibodies.

Increase sensitivity with high sensitivity HRP substrates, or try optimizing current blocking buffer conditions. We transfer our gel to PVDF overnight at 12-15V, block in TBS-T with 5 dried milk for 1.5. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. The ladder seems to transfer fine because the pre-stained marker shows up quite well on the membrane.

Having difficulty detecting low abundance proteins? The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection.

Protein ladder on western blot